WHAT IS DEPTH OF FOCUS/DEPTH OF FIELD?

1.Overview of depth of focus/imaging depth of field

In a regular lens, only one point is completely in focus.

(Special lenses for precision measurements such as telecentric lenses are excluded)

There are areas that are less out of focus before and after the point that is perfectly in focus.

This is called depth of field.

If you move away from a point that is completely in focus, the point will gradually become blurred.

By narrowing the optical path, you can reduce the level of blur.

However, because the image becomes darker when stopped down, it cannot be used with very high magnification lenses.

2.Depth of focus/depth of field of the microscope

 

Pictured below is our full-aperture microscope, the MS200PC3 (20-110x).

絞り付マイクロスコープ  

Using a microscope with this aperture, we will compare the images when the lens is wide open and when the lens is closed.

(Reduce aperture to increase depth of field.)

Tilt the 0.5mm pitch glass scale at 45 degrees and observe it from above.

 

0.5mmピッチのガラススケールを観察 0.5mmピッチのガラススケールを観察

(1) Depth of focus/depth of field at 50x

<When opening the aperture> <When narrowed to maximum>
絞りを開放にした時 最大に絞った時

How much focus there is depends on each individual

Since it is tilted at 45 degrees, multiplying by 1/1.41 gives the depth of stroke.

If you judge 4 steps (= 2 mm) to be correct then the depth of field is 2 mm x (1/1.41) = 1.42 mm.

Tilt the board at a 45 degree angle and observe it at 50x magnification.

(1.6 mm x 0.8 mm electronic components are spaced 1 mm apart.)

 

 

<When opening the aperture> <When narrowed to maximum>
絞りを開放にした時 最大に絞った時

As reference, I also confirmed at 100x.
Because of the high magnification, the glass scale was changed to a distance of 0.2mm.

 

<When opening the aperture> <When narrowed to maximum>
絞りを開放にした時 絞りを最大に絞った時

Please note that if you close the aperture, the lens will become darker and the resolution will decrease.

 

3.Depth of focus/technique to increase depth of field

◆Explanation: About the focal depth/depth of field of the lens

 

Before introducing this trick, I want to explain the lens’s depth of focus.

Depth of focus is also expressed in DOF (Depth of Focuse).

Case in air(N=1)

DOF=(0.55/(2×NA²))+(1/M×K/NA)

Formula explanation)

 

  • The first item is “an item defined by resolution.”
  • The second item “K” is “a constant determined by the resolution of the observer’s eye” and varies from person to person.
  • “M” is the “overall magnification of the lens”.

What we can say from this is that the only way to increase depth of focus is to decrease magnification or decrease NA.

At the same magnification, the only option is to reduce NA.

 

The photos below are from the same manufacturer and product line, with the megapixel-compatible lens on the left and the lower-resolution all-purpose lens on the right.

被写界深度比較

When comparing lenses with the same magnification and focal length, general purpose lenses have a deeper depth of focus.

The only way to reduce NA with the same lens is to narrow the aperture.

If the lenses are different and the magnification is the same, the longer the focal length, the lower the NA, and the deeper the depth of focus.

◆Introduction to tips: “Aperture” and “Digital Zoom”

However, because both “aperture” and “digital zoom” tend to reduce resolution, we recommend using a 4K (8 million pixel) camera and a lens with equivalent high resolution. response.

 

I attached a 10 million pixel compatible 50mm lens to a 4K (8 million pixel) camera and attached a close-up ring for 50x macro photography for comparison.

 

固定焦点レンズで作ったマイクロスコープ

 

Take a photo of the same board as above under the same conditions.

(1) AT 50X, ADJUST DEPTH OF FOCUS/DEPTH OF FIELD JUST BY CHANGING LENS APERTURE

 

<When opening the aperture> <When narrowed to maximum>
絞りを開放にした時 絞りを最大に絞った時

(2) After adjusting the digital magnification to 50x, adjust the depth of focus/depth of field using the lens aperture.

<When opening the aperture> <When narrowed to maximum>
絞りを開放にした時 絞りを最大に絞った時

(3) Benefits when using the procedure

Since it uses a fixed focus lens, zoom is not possible.

However, unlike macro zoom lenses, the working distance is not fixed, so there is flexibility in terms of W.D. longer, shrinking the system and nuances of depth of field.

Depending on lens selection, any (fixed) magnification can be set from 5x to 50x.

4.Conclude

The area in front and behind a point that is completely in focus and slightly out of focus is called depth of field.

How realistic it is depends on each individual.

By using a combination of “aperture” and  “digital zoom”, a deep depth of focus/depth of field can be achieved even with a simple microscope built with a lens fixed focal length.

 

WANT TO PERFORM LOW MAGNIFICATION OBSERVATIONS WITH A COAXIAL ILLUMINATED USB MICROSCOPE

Z500CS

 

Our Z500CS coaxial illumination USB microscope comes with a 1.5x auxiliary lens as standard.

 

This time, we tried to observe the metal parts of the USB memory.

同軸照明USBマイクロスコープで低倍率観察02

 

 

 

同軸照明USBマイクロスコープで低倍率観察01  

Observe with 1.5x secondary lens (basic specifications)

 

Focal length, magnification and field of view are:

・Focal length: 52mm

・Visibility range(at 65x): 5.2mmx3.9mm

・Visibility range(at 390x): 0.8mmx0 .6mm

 

 

 

 

 

同軸照明USBマイクロスコープで低倍率観察03   同軸照明USBマイクロスコープで低倍率観察04  
  Seen like this at 65x   Seen like this at 390x  

 

 

 

If you want to observe at low magnification, remove this 1.5x auxiliary lens to reduce magnification.

 

同軸照明USBマイクロスコープで低倍率観察05  

Remove the 1.5x secondary lens. Focal length, magnification and field of view at this time are:

・Focal length: 95mm

・Visibility range (at 45x): 8.0mmx6.0mm

・Visibility range(at 270x): 1.2mmx0.9mm

 

 

 

 

 

同軸照明USBマイクロスコープで低倍率観察06   同軸照明USBマイクロスコープで低倍率観察07  
  Seen like this at 45x   Seen like this at 270x  

 

 

 

If you want to observe at even lower magnification, attach the 0.75x auxiliary lens to reduce the magnification.

 

同軸照明USBマイクロスコープで低倍率観察08  

Attach the 0.75x auxiliary lens At this point, the focal length, magnification, and field of view are:

・Focal length: 113mm

・Visibility range (at 35x): 11.3mmx8.5mm

・Visibility range (at 210x): 1.7mmx1.2mm

 

 

 

 

 

同軸照明USBマイクロスコープで低倍率観察09   同軸照明USBマイクロスコープで低倍率観察10  
  Seen like this at 35x   Seen like this at 210x  

 

In this way, the minimum magnification of 35x (0.75x) cannot be used because the coaxial illumination becomes unbalanced.

Therefore, the lowest practical magnification is to remove the standard 1.5x auxiliary lens and observe at a minimum 45x magnification.

 

 

For details about the “coaxial illumination USB microscope” and “0.75x auxiliary lens” used this time, please see the product page below.

 

Z500CS  

Z500CSLT coaxial illumination USB microscope

 

 

 

 

 

0.75倍補助レンズ Z-0.75  

0.75x auxiliary lens (for FZ/SDS-FZR series microscopes) Z-0.75

 

COMPARE THE RESOLUTION BETWEEN NSH LENS AND USH LENS

Comparison at 1400x (X5) is near the highest magnification of the NSH lens

■Features of Lens

When using extremely high magnification lenses (over 1000x), the difference in lens performance becomes apparent near maximum magnification.

・Blurred edges (aberration)

・Color fading (chromatic aberration)

 

Depending on the subject, edge blur (aberration) and color blur (chromatic aberration) can have a significant effect on appearance.
→If this level is reduced to the maximum, the lens price will increase significantly.
→On the other hand, depending on the subject, costs can be significantly reduced.

■Examples have little impact

When examining samples on silicon wafers, the edges may be slightly blurred but are still fully visible. (Photo below)

Lens used: NSH Lens (multi-purpose lens)

 

汎用レンズの解像度

 

Lens used: USH Lens (high resolution lens)

高解像度レンズの解像度

■Examples have many influences

Edge blurring and chromatic aberrations are also present. Although the presence or absence of foreign objects can be determined, detailed observations cannot be made.

Lens used: NSH Lens (multi-purpose lens)

汎用レンズの解像度

 

Lens used: USH Lens (high resolution lens)

高解像度レンズの解像度

HOW TO CONNECT THE CAMERA WITH MONOCULAR (MICROSCOPE)

There are various types of monoculars.
Some have removable eyepiece lenses, while others cannot.

■If the eyepiece lens cannot be removed
When the eyepiece diameter is φ30mm to φ32mm and made of hard material such as resin

 

接眼部のレンズが外せない場合

 

You can attach a camera by connecting an adapter lens like the one below to the eyepiece part.

 

アダプタレンズをアイピース部分の接続

 

可変倍率カメラアダプタレンズ BA-A1835 Variable magnification camera adapter lens BA-A1835
   
カメラアダプタレンズ BA-A35 Camera adapter lens BA-A35

 

■When the eyepiece lens can be removed
When the inner diameter of the straight cylinder after removing the eyepiece is 23.2 mm (JIS standard)

 

接眼部のレンズが外せる場合

 

You can connect a C-mount camera by removing the eyepiece and inserting a relay lens in its place as shown below.

 

リレーレンズを挿入するとCマウトカメラの接続が可能

EQUIPMENT RECORDING PICTURES BEFORE AND AFTER TRIGGER

■RECORDER

A device that can record images from before an event occurs is called a skipback recorder.

The images below can be saved before and after an external trigger signal is input.
You can also extract the images you need as still images.

スキップバックレコーダー

 

 

■USB CAMERA + SOFTWARE

 

Also, if you can use a PC without using such equipment, you can record videos before and after the trigger occurs by using a USB camera or GigE camera and equipment monitoring drive recorder software.

 

FIXTURE USING TRIPLE HOLE (1/4 INCH SCREW)

The tripod hole is used for relatively many purposes other than cameras.

The strobe lighting shown below has a tripod hole.

 

三脚穴 三脚穴
   

 

In addition, we will introduce fixing devices that use a tripod hole.

カメラの三脚には固定

■A flexible arm (bellows) type fixture that can be clipped onto the edge of a desk.
フレキシブルアーム(蛇腹)タイプの固定具

 

変わった固定具 変わった固定具
   

 

■This is our edge fixed stand with a 3D arm.

 

3Dアーム付きエッジ固定スタンド

 

It can also be installed on our Easy Arm.

 

イージーアーム イージーアーム
It is an arm with three joints that can move freely.  
   
イージーアーム(バーLED・カメラ・モニタ用)KA-N Easy arm (For bar LED, camera, monitor) KA-N

METHOD TO OBSERVING LARGE METAL

We sometimes receive requests to see on-site the composition of metal products that are too large to be cut out. In that case, we take the following two points into consideration and propose a custom-made product like the one in the photo below:
– Fixed magnification to reduce weight and cost. (Can be changed using the objective lens at the tip)

– Insert a cutout in the stand to directly observe the object.

大きな金属の場合の観察方法 大きな金属の場合の観察方法
Of course, coaxial illumination is used to observe the metal composition.
   
Small simple metallurgical microscope
KKKI-STD6-130DN
   
単眼式同軸測定顕微鏡 For a monocular coaxial measuring microscope like the one on the left.
Connect a camera to reduce weight

 

 

カメラはUSBタイプ、ハイビジョンタイプ等から選べます The camera is USB type, you can choose from high-definition types, etc.

STANDS FOR OBSERVING LARGE OBJECTS

We have a track record of manufacturing custom-made stands such as the ones below.

 

大型対象物が観察しやすいスタンド1

 

 

It is a manual type, but an electric type is also available.

 

大型対象物が観察しやすいスタンド2

 

 

It is large enough to fit A4 size paper.

 

大型対象物が観察しやすいスタンド3

 

大型対象物が観察しやすいスタンド4

 

大型対象物が観察しやすいスタンド5

 

 

You can observe every corner of the table.

 

大型対象物が観察しやすいスタンド6

You can create deep pockets by creating a structure like the one above.

HOW TO USE AN ULTRA HIGH MAGNIFICATION MICROSCOPE (NSH500CSU) USING TRANSMITTED LIGHTING

The NSH500CSU comes standard with a simple XY panel but does not support transmitted lighting, so we will cover how to install a transmitted lighting system.

 

◆Method 1
How to attach the rubber feet to the RD-95T and place it on the standard XY platform (TK100)

RD-95Tにゴム足を取付け、標準付属のXYステージ(TK100)に載せる方法

 

Light can be easily passed through without processing, but the size of the object is limited to the size (φ95) that can be placed on the RD-95T.

Because it has rubber feet, it will not slip, but the lamp body may move due to impacts such as being hit by your hand.

 

◆Method 2
How to switch to the XY turntable simply, remove the observation plate and install the RD-95T

 

回転式簡易XYステージに変更し、観察板を外しRD-95Tをはめ込む方法

 

Since the RD-95T can be mounted to the XY table, it can be fixed to some extent.
Since it is necessary to route the cable to the outside, it is necessary to drill a hole about φ10 in size in the base.
We can also drill holes for free before shipping.

 

 

For details on the products introduced this time, please see below.

超高倍率USB マイクロスコープ NSH500CSU

NSH500CSU ultra-high magnification USB microscope

   

 

 

 

回転式簡易XYテーブル TK180-K

Simple XY rotary table TK180-K

   

 

 

 

透過照明 RD-95T Transmitted lighting RD-95T

RECORDING DEVICE

There are many low-priced hard disk recorders on the market.
The following products come with a monitor, and the internal hard disk can be replaced with a commercially available size.
Long-term recording is possible for up to 2 months.
It has four video (NTSC) input terminals.

 

長時間動画を保存する機器

 

Also, if you can use a PC without using such equipment, you can record long videos by using a USB camera or GigE camera and long-term recording software.

 

 

GLASS SCALES

Various types of glass scales are also available.

The photo below is of Shodensha’s glass scale.

Calibration glass scale GS-4SQ

 

校正用ガラススケール GS-4SQ

 

When calibrating our microscope, the grid type is easier to adjust than the cross display. Furthermore, it would be convenient to have several square sizes to suit various magnifications.

The glass scale mentioned above is perfect for this purpose.

ACCESSORIES TO REDUCE HALATION

There is a way to use white V-shaped blocks.

 

白色のV字ブロック
 
I tried observing a metal cylinder using a V-shaped block. (Uses a white resin V-shaped block)

 

白色の背景で観察
V字ブロック使用して観察
<Observation on a white background>
Simple reflected light causes halation.

 

<Observation using V-shaped block>
By using V-block Illumination from the side is also provided.

 

 

V字ブロック The size of the V-shaped block is 80mm x 50mm x 30mm

 

RELATIONSHIP BETWEEN DEPTH OF FIELD AND OPERATIONAL DISTANCE

To increase the depth of field, there are two typical methods below

(1) Increase depth of field.

(2) Narrow the optical path

 

This time we will explain about (1)

■Relationship between depth of field and operating distance

Below is a table of specifications for lenses from the same manufacturer, same series, and same magnification (X4).

W.D. The longer it is, the deeper the depth of field.

 

レンズのスペック表

 

 

■Measure the actual depth of field thanks to the difference in operating distance

Tilt the 0.2mm pitch glass scale at 45° and observe it from above.
Comparative shooting with lenses from the same manufacturer and lens series with different operating distances.

 

 

真上からから観察

 

 

(1) Observe with x6 optical magnification lens with operating distance of 40mm

 

作動距離40mmのレンズで観察

 

If we judge that the focus is on a scale (0.2 mm), then the depth of field is 0.2 mm x (1/1.41) = 0.14 mm.

 

 

(2) Confirm with a lens with optical magnification X6 and operating distance of 110mm

 

110mmのレンズで確認

 

If we judge that the focus is on two scales (0.4 mm), then the depth of field is 0.4 mm x (1/1.41) = 0.28 mm.

 

PHOTO STAND (COPY STAND)

When using a digital camera to photograph paper materials, books, or other three-dimensional objects that cannot be copied, it is convenient to have a copy stand.

There are various sizes and prices on the market, but the ones below are relatively affordable and allow you to take photos up to A4 size.
The size is 450x450mm and the pillar height is 550mm.

 

撮影台(コピースタンド)

 

We also provide stands and lights that can be used like pseudo-copy stands that allow you to observe A4 size documents exactly.

 

低価格カメラスタンド GR-STD1-115 低価格カメラスタンド GR-STD1-115

 

HOW TO USE A HANDLE MICROSCOPE

When using a handheld microscope, there are 3 following vital notes: 

1. Display speed

When taking pictures with a handle microscope, the screen inevitably shakes.
If the display speed is slow, the “shaking” phenomenon will not stop.

2. Power source

A microscope needs two power sources: the camera and the light source.
Dedicated machines may be provided with a USB port.

3. Adjust brightness

Since one hand is busy, I think it would be easier to use if there was an automatic exposure mode.

DinoLight products are available as special handheld machines with a wide range of products and low prices.

 

DinoLight

 

Depending on the combination, some of our products can also be used as hand tools (Since this is a combination product, it is not listed in the catalog as a set, so please contact us.)

・1.3 million pixel USB3.0 camera (global shutter USB3.0 camera with fast display speed)
・Low magnification SG2 lens (small, small diameter lens for ergonomic users) 
・16-light ring light (can be directly connected to the lens and powered by a PC USB port)

 

Dimensions as shown below

 

 

Both the camera and light can be powered from a PC. (No separate power supply required.)

 

ハンディマイクロスコープ

 

Because lens are so small and narrow, the zoom ratio is not wide.

 

ハンディマイクロスコープ

Is image processing necessary as pre-processing for image testing?

● Issues with Automated Visual Inspection

In recent times, many users have incorporated automated visual inspection using image-based methods.

  • Pixel-check type automated visual inspection,
  • Pattern-check type automated visual inspection
  • Automated visual inspection with AI embedded in the above types.

While there are various methods, some users encounter challenges where the automated visual inspection fails to detect and overlooks instances marked as “NG” (Not Good). Upon investigation, it is often found that the NG elements are not clearly visible in the inspection images.

● Necessity of Image Processing

In such cases, reconsideration and reconstruction of hardware elements such as:

  • Camera resolution
  • Camera performance
  • Lens performance and magnification
  • Illumination methods are often required. However, even after revising the hardware, successful detection can be challenging.

As mentioned earlier, the fundamental principle is that even humans, software, or AI cannot detect what is not visible. In fact, at this point, software and AI may be inferior to humans. In such instances, there arises a need for image processing as a preprocessing step.

Instead of directly streaming raw acquired images to the inspection software, it is preferable to apply image processing as a preprocessing step before streaming the processed images to the inspection software. While this may increase tact time (inspection time), it is an effective method when detection without preprocessing is challenging.

● Types of Image Processing

Image processing includes various techniques such as binarization, contrast correction, brightness correction, color correction, color limitation, smoothing, edge enhancement, contour enhancement, noise reduction, sharpening, and, in a broader sense, resizing, measurement, line generation, and more.

● How is Image Processing Performed?

Typically, static images are saved, and image processing is carried out using an image processing unit.

● Can Image Processing be Done in Live View (Real-Time)?

The answer is yes. If the microscope’s display software has image processing options in the view function, you can perform image processing in real-time during live view and then save the static image. However, such capabilities are often found in high-end microscopes, requiring a substantial investment.

 

 

 

印刷した文字を観察
Observe the printed characters
 

 

ファイバーの端面を観察
Observe the fiber end face
 

 

クリームの粒子を観察
Observe cream particles

 

CHECKING VISUALITY WITH A MICROSCOPE

CHECKING VISUALITY WITH A MICROSCOPE

Many of the examinations performed using a microscope on the inspection line are extremely tiring and place a significant burden on the examiner. Therefore, many people are thinking about replacing it with a small microscope. However, there are many types of microscopes and if you don’t choose the best one, you may be creating an unnecessary burden on yourself.
There are three points to keep in mind when replacing a microscope with a mini microscope:

THREE IMPORTANT POINTS FOR REPLACEMENT

① CHOOSE A MODEL WITH A HIGH FRAME RATE

The microscope displays images on a PC or monitor.
If the frame rate is low, the projected image will not move smoothly, causing additional tension.
Frame rate is expressed as a number, but the number that makes people feel uncomfortable is around 50 to 60 fps.
When the speed drops below 30fps, the feeling of discomfort becomes noticeable.
PC-connectable models often have low frame rates due to USB communication speed issues.
Therefore, we recommend that you choose the screen direct connection type.

②Choose a model with good color reproduction

The appearance will vary depending on the camera used for the microscope.
For this reason, there are some items with good color reproduction and others with not so good.
If the colors don’t look good, the human brain will feel uncomfortable and this will lead to stress.
Even if a red object looks slightly orange, it can make you feel uncomfortable and stressed.
If you choose a camera with full resolution, the color reproduction is relatively high, so you can get clear images.

③SELECT A MODEL WITH WIDE DYNAMIC RANGE

I don’t think you’ve heard the term dynamic range often.
Briefly explained, when you illuminate a bright object and a dark object at the same time, if you adjust the brightness of one object, the other object may appear blown out or dim.
To eliminate this phenomenon, a camera with a wide dynamic range is needed.
Dynamic range is a state that a camera has but it also varies depending on the camera.
Compared to conventional cameras, the human eye is very good and has a very wide dynamic range.
The main reason why things look different to the human eye and through a camera is often due to differences in dynamic range.
There are models that emphasize wide dynamic range, so I think it’s best to choose one of those.

THE RECOMMENDED MODEL IS:

We also have car models that meet all 3 points above.
Full high definition microscope
Frame rate = 60fps
Color reproduction = Very clear resolution thanks to full high definition resolution
Dynamic range: Wide with HDR function (high dynamic range)
We also have demo machines, welcome to try them out.

THE POOR REPRODUCIBILITY OF THE PRINTER

There may be instances where the captured images cannot be printed with the desired color reproducibility.

When the resolution of the captured images is sufficient, the printer’s performance may be influencing the outcome.

<USING GENUINE PAPER>

Using genuine paper from the printer manufacturer or paper closely resembling to them.

<USING A 6-COLOR TYPE (OR MORE)>

Color printers cannot reproduce colors lighter than the set ink colors. Equipping the printer with lighter colors such as light cyan, light magenta, gray, etc., allows for subtle adjustments in color variations. While standard printers often have 4 colors, it is advisable to use 6 colors (or more) when printing images.

<USING A HIGH QUALITY PRINTER TO PRINT PHOTOS>

If you use a high-quality photo printer like the one below, the quality of your printed images will be improved.

 

プリンター

ABOUT SURGE COUNTERMEASURE

Surges, stemming from various factors, refer to abnormal voltages and the resulting abnormal currents.

Causes include “lightning surges” induced by lightning and “switching surges” resulting from the ON/OFF of the power supply.

Concerning “lightning surges,” power companies and communication providers implement diverse countermeasures, and specific surge-protective devices, such as surge tables, are available for purchase.

 

雷サージ用のテーブルタップ

 

“Switching surges” pertain to surges occurring during circuit opening and closing. Caution is advised when large-capacity motors, generators, high-voltage circuits, solenoids, etc., are connected to the same power line.

I

f power supply circuits frequently experience damage, abnormal operations occur, or similar issues arise, surge protection may be necessary. Separating power lines can be a straightforward solution, and various surge protection devices are also available for purchase.

MICROSCOPE USING A SINGLE-LENS REFLEX CAMERA

Combining a step-down ring with Nikon’s telephoto lens (f = 300mm), attach an M26 microscope objective lens to the end.

 

ステップダウンリングを組みあわせてM26の顕微鏡用対物レンズを取り付け

 

Achieving imagery and observing a clear visual representation, the lens-side distance adjustment dial operates at a fine-tuning level.

取付例

 

Projecting a glass scale with a 0.2mm pitch, the horizontal field of view is 0.4mm, allowing for a magnification of approximately 800 to 1000 times.

 

0.2mmピッチのガラススケールを映してみます

 

It is worth noting that this method is applicable even with standard lenses (approximately f = 50mm). While the magnification decreases, it is adequately accommodated with ring illumination.

 

標準レンズ(f=50mm程度)でもこの方法は使えます

The focal length is around 40mm, shorter than that of a dedicated microscope.

LONG DISTANCE SHOOTING / ZOOM IN WITH A SINGLE – LENS REFLECT CAMERA

The method to connect SLR lenses to camera C for long-distance and magnified photography involves the relatively straightforward application of a C-mount to F-mount adapter.

 

Cマウント⇒Fマウントの変換アダプタ

 

Utilize the aforementioned adapter with Nikon’s telephoto lens (f =300mm) and connect it to DN3V-130.

 

DN3V-130に接続

 

The minimum distance is approximately 3 meters.

Observe the distant view at 3 meters, as depicted in the image below: 60mm×45mm.

 

最至近距離は3m程度

LONG DISTANCE SHOOTING / ZOOM IN WITH A SINGLE – LENS REFLECT CAMERA

The method to connect SLR lenses to camera C for long-distance and magnified photography involves the relatively straightforward application of a C-mount to F-mount adapter.

 

Cマウント⇒Fマウントの変換アダプタ

 

Utilize the aforementioned adapter with Nikon’s telephoto lens (f =300mm) and connect it to DN3V-130.

 

DN3V-130に接続

 

The minimum distance is approximately 3 meters.

Observe the distant view at 3 meters, as depicted in the image below: 60mm×45mm.

 

最至近距離は3m程度

HOW TO ACCURATELY MEASURE SIZE WHEN OBSERVING BY A DIGITAL MICROSCOPE

3D arm is convenient for observation with digital microscope.

 

TG-3D3  

3D arm of microscope

 

<Image>

3Dアームで斜め観察時のコツ1
Take photos right next to the microscope

 

3Dアームで斜め観察時のコツ2   3Dアームで斜め観察時のコツ3
Take photos directly from the front    

 

 

3Dアームで斜め観察時のコツ4

 

Therefore, even when observing at the same magnification, the same vertical calibration value cannot be used. When measuring by oblique observation, it is necessary to correct for such conditions.

 

Summary

 

For accurate measurements by oblique observation, calibration must be performed with the microscope tilted at a 45 degree. However, if you are tilting two axes (tilting not only left and right but also toward you), you need to consider both axes when calibrating.

For details on the “3D arm” and “measuring software” used in this measurement, please see below pictures.

 

TG-3D3  

3D arm for microscope

TG-3D3

 

 

 

 

High-performance image processing measurement software

MFship

UTILIZING THE LENGTH MEASUREMENT FUNCTION OF A MICROSCOPE FOR QUALITY CONTROL

In this article, we shall present the “Points to Consider,” “Usage Guidelines,” and “Recommended Microscopes” when utilizing the length measurement function of microscopes for quality control.

POINTS TO CONSIDER

While some microscopes have the capability to measure length, two operational issues may arise:

(1) The microscope itself is not a traceable measuring instrument.

(2) Calibration, except for some high-end models, must be performed by the user. (Errors may occur due to user operations.)

USAGE GUIDELINES

When used for quality control purposes, there is no issue with internal calibration. However, clarification is needed regarding the standards used in internal calibration. In other words, implementation is feasible if we can meet the following three conditions:

  • Using a microscope with reproducibility capability.
  • The reference device used to check the accuracy of the microscope is transparent.
  • Standards with traceable capabilities are available.

We have customers who have obtained ISO9001 certification and continue to use our products.

RECOMMENDED MICROSCOPES

When utilizing the length measurement function of microscopes for quality control, we recommend the following:

 

Microscope model CT200HD:

 

 

◆ Rare in the mid-range product line. Lens-camera interlocking system

The camera collects data from the lens and automatically utilizes the calibration data stored in the camera’s internal memory:

・ No adjustment required by end-users.

・ Values remain unchanged regardless of end-user operations.

◆ Providing special options with accurate measurement scale

May include calibration certificate and test results table for accurate measurement scale. (Charges)

 

校正証明書1   校正証明書2

 

 

 

Microscope model CT200HD:

 

  Microscope model CT200HD-H (Medium Magnification, Automatic Calibration).

 

寸法測定(自動校正)マイクロスコープ(高倍率) CT200HD-H  

Microscope model CT200HD-H (High Magnification, Automatic Calibration).

OBSERVING BURRS INSIDE A HOLE USING TRANSMITTED ILLUMINATION METHOD.

Utilizing transmitted illumination to observe burrs and foreign objects within apertures.

We often receive requests to inspect burrs and foreign objects inside holes using transmitted illumination.

When the target object has a certain “thickness,” a bit of technique is required.

We conducted an examination by creating a hole with a diameter of φ5mm in a 12mm-thick aluminum plate and capturing images.

 

 

穴の中のバリや異物を観察

 

The upper, middle, and bottom surfaces in the above image have foreign objects adhered to them.

 

トップ(表面)、ミドル(中間)、ボトム(底面近く)

 

 

To detect all of these, the use of a lens with aperture control is necessary.

 

 

 

トップの焦点を合わせる

 

ミドルに焦点を合わせる

 

 

■ When using an open-aperture lens

Observing the top surface with the lens opened and focused yields the following results.

 

開放レンズを使った場合

* The middle and bottom become nearly imperceptible.

When focusing on the middle using an open-aperture lens for observation, the results are as follows.

 
 

 

開放レンズで中間に焦点を合わせ観察すると下記のようになります

*The top is visible but appears slender, while the bottom is in a highly indistinct state.

 

■When employing a macro lens with aperture control…

絞り付きのマクロレンズを使った場合

 

Capture the image with the aperture narrowed as much as possible.
  (Compensate for the resulting darkness with additional illumination, and keep the camera gain around 90% rather than at its maximum.)

 

*Both the top (surface) and middle (interior) as well as the bottom (near the base) can be adequately observed.

 

トップ(表面)・ミドル(中間)もボトム(底面近く)ともに十分に観察できます。

 

  • In conclusion,

To observe burrs and foreign objects within the hole, it is essential not only to use transmitted illumination but also a lens with aperture control that can deepen the depth of field.

“SUITABLE MICROSCOPES” AND “UNSUITABLE MICROSCOPES” FOR DIMENSION MEASUREMENT

Many microscopes on the market have a size measurement function. However, some types are still difficult to use. When using the measuring function, a microscope can be said to be suitable for measuring dimensions if it has the following two characteristics:

(1) Zoom lens.

(2) Latching function.

Below we will introduce each characteristics in detail.

(1) Zoom lens:

There are two types of lenses that can continuously change magnification:

  • “Variable magnification lenses” are not suitable for measuring because the focal length changes significantly when magnification is changed.
  • “Zoom lens” are suitable for measuring because the focal length remains almost the same even when magnification is changed.
(Maximum magnifiaction)  

(Minimum magnifiaction)

寸法測定に向き・不向きマイクルスコープ01   寸法測定に向き・不向きマイクルスコープ02

<Suitable for measurement> Zoom lenses (except our SG series)

(Maximum magnifiaction)   (Minimum magnification)
寸法測定に向き・不向きマイクルスコープ03   寸法測定に向き・不向きマイクルスコープ04

(2) Latching function:

When measuring dimensions, the ability to reproduce conditions is important. If the optical magnification (scale) is marked on the lens and each scale has a latching function, inter-operator variation is eliminated and reproducibility is maintained.

 

<Not suitable for measurement> Products without latching function

 

Low-cost microscope series (sG series)

 

Low magnification microscope (LRS series)

High magnification microscope (LRA series)

寸法測定に向き・不向きマイクルスコープ05   寸法測定に向き・不向きマイクルスコープ06

 

<Suitable for measurement> All other product lines have latching function.

寸法測定に向き・不向きマイクルスコープ07

 

(Note) If you want to measure with a low magnification microscope as mentioned, our “low magnification microscope” (LRS series) is not suitable for size measurement (Because reproducibility cannot be achieved). Although the magnification cannot be lowered with the LRS series, if you attach the “0.5x auxiliary lens” to the TG series, it can be used as a low magnification microscope for reproduction.

 

TG series   Auxiliary lens on 0.5X
USBマイクロスコープ 寸法測定に向き・不向きマイクルスコープ09

Microscope TG500CS

 

 

Auxiliary lens on 0.5X

Although the TG series could not achieve as low magnification as the LRS series, it can maintain a field of view as wide with two dimes and perform highly reproducible measurements.

 

寸法測定に向き・不向きマイクルスコープ10

 

 

LONG-RANGE (EXTENDED WORKING DISTANCE) MICROSCOPE

We occasionally receive requests expressing the desire to increase magnification with an extended working distance.

Typically, increasing magnification results in a shorter focal length.

Allow me to present options classified under the category of long-range types.

 

■ Long-Range (Extended Working Distance) Microscope (LRA Series)

 

Our extended working distance microscope not only incorporates a 10x zoom function but also achieves an extended working distance seamlessly.

The focal length can be adjusted freely within the range of 200 mm to 400 mm.

 

長距離(長作動)マイクロスコープ (LRAシリーズ)

 

(1) The field of view at maximum magnification when the focal length is set to 250 mm.

 

In the horizontal direction, it measures 6.7 mm, equivalent to an approximate magnification of 70 times.

 

焦点距離を250mmに設定した時の最大倍率時の視野

 

高機能長距離ハイビジョン マイクロスコープ
(スタンドタイプ)LRA200XM-S

High-performance Long-distance High-Definition Microscope (Stand type)

LRA200XM-S

 

 

(2) Resolution at Maximum Magnification with a Focal Length Set to 250 mm

 

The resolution, as reflected by a glass scale used for resolution checks, is as follows.

 

焦点距離を250mmに設定した時の最大倍率時の解像度

 

(3) Advantages and Disadvantages

 

While the presence of a 10x zoom function and the ability to vary magnification at the same distance represent significant advantages, there is a slight reduction in resolution.

The field of view at maximum magnification with a focal length set to 250mm→400mm is as follows.

 

メリットとデメリット

 

 

■ ƒ=75mm Fixed-Focus Lens Application

 

By adding a 2x rear converter and a 15mm close-up ring to a 75mm telephoto lens, it can serve as an alternative to the Long-Working Distance Microscope (LRA series).

 

f=75mm 固定焦点レンズの応用

 

(1) Field of View when the focal length is set to 250mm

Without a zoom function, once the focal length is decided, the magnification is determined. The horizontal field of view is 8mm, resulting in approximately 60x magnification.

 

焦点距離を250mmに設定した時の視野

 

 

(2) Resolution at maximum magnification when the focal length is set to 250mm

 

When projecting a glass scale for resolution checks, it appears as follows.

 

焦点距離を250mmに設定した時の最大倍率時の解像度

 

 

(3) Pros and Cons

 

While the focal length can be adjusted, once determined, it fixes the magnification as well. Without a zoom function, the lens has fewer elements, resulting in a brighter image and higher resolution compared to the aforementioned Long-Working Distance Microscope (LRA series).

In a direct comparison at maximum magnification, it slightly falls short compared to the Long-Working Distance Microscope (LRA series). The price is lower due to the absence of a zoom function.

When the focal length is set to 250mm→400mm, the field of view is as follows.

 

メリットとデメリット

 

 

■ High-Resolution, High-Magnification Zoom Lens

 

There are specialized lenses with high resolution, high magnification, and zoom functionality available through our products. For more details, please inquire.

It is capable of achieving 420x magnification at a focal length of 200mm.

 

高解像度・高倍率ズームレンズ

How to observe polarized light with a microscope

When conducting polarized observation, the efficacy is heightened by incorporating polarization filters on both the emitting and incident light sides. Subsequently, the polarization intensity is adjusted by rotating either of the polarization filters.

 

 

Our company’s microscopes, specifically the TG and FZ series, are equipped to facilitate polarized observations by directly affixing light-emitting diodes (LEDs) to the lens.

 

偏光観察

 

<When diminishing magnification by attaching an auxiliary lens>

Due to the concurrent placement of auxiliary lenses and incident-side filters, their simultaneous attachment is precluded.

 

補助レンズ装着時

 

 

In this scenario, the utilization of our LED Angle (LED-A2) enables polarized observations.

 

 

LEDアングル使用時

 

 

LEDアングル使用時

LEDs are affixed beneath the angle.

(Caution) The installation space for the angle cannot be secured unless the focal length is elongated. Consequently, it can only be utilized when employing auxiliary lenses to reduce magnification.

METHODS FOR EMPLOYING HIGH-MAGNIFICATION MICROSCOPES AT EXTENDED DISTANCES

Introducing Approaches to Achieve High Magnification Levels Beyond 200x with a Focal Length of Over 100mm.

 

Fixed Magnification Macro Lenses

 

 

固定倍率のマクロレンズ

 

 

 High Magnification Zoom Lenses

 

・Our NSH lenses feature specialized objectives, providing 2x or 5x.

 

高倍率・ズームレンズ

 

オプション 2倍対物レンズ

Option: 2X Objective Lens QM Plan Apo L2 (2X)

 

Option: 5X Objective Lens QM Plan Apo HL (5X)

 

Magnification, constituting high-magnification, high-resolution zoom lenses.

 

This is an exceptionally unique lens.
Achieving high magnification and long working distances while ensuring elevated resolution.

 

 

高倍率・高解像度・ズームレンズ

 

 

f=100mm yields 900x magnification

f=200mm provides 420x magnification

マイクロスコープと実体顕微鏡の違い

実体顕微鏡とマイクロスコープにはそれぞれ、メリット、デメリットがあります。
それぞれの特徴を理解して使い分ける必要があります。

 

本来、実体顕微鏡も英語で表記すれば、マイクロスコープとなります。
業界によっては、顕微鏡をマイクロスコープと呼ぶ場合もありますが、今回は単眼のデジタルマイクロスコープをマイクロスコープとしてご説明します。

 

目次

1. 特徴の違い

2. 倍率の違い

3. 主な用途の違い

 

 

 

1. 特徴の違い

・実体顕微鏡の特徴

大きな違いは、実体顕微鏡は2光路設計になっていることです。
右と左で独立した光路となります。

 

2光路設計

 

右と左で視野も異なります。
下記のコインを立てて、実体顕微鏡で観察すると

 

コイン

 

コイン

 

この異なった視野を観察者が一つの映像として観察します。
人間の目が2つあるのと同じです。

 

これによるメリットは、対象物が立体的に見えます。
遠近感もわかるので、加工作業をされる方は実体顕微鏡が必要です。
デメリットとして、使う時に少しコツが必要です。
(初めて使う方は、映像が1つにならず、戸惑うと思います。)

 

3眼式の実体顕微鏡の場合、カメラポートは左右どちらかの映像となります。
(映像も斜視となります。)

 

デメリットとしては、高倍率の観察ができません。
(製造時に左右の微妙な調整が必要な為です。)
倍率は比較的低倍率となります。ズーム比もそれ程大きくありません。
汎用的なもので、10~50倍程度、高倍率タイプでも100倍程度が実体顕微鏡の限界となります。

 

また、長時間の作業では作業者のストレスが大きくなります。
目幅調整、視度調整 等、観察者個人ごとの調整が必要です。

 

左右で視野が異なるので、正確な位置決め、2次元の寸法測定には不向きです。
上記の用途で顕微鏡を使う場合は、単眼顕微鏡を使います。

 

単眼顕微鏡

 

 

・マイクロスコープの特徴

基本的には単眼レンズとなります。

 

マイクロスコープ

 

人間が片目で物を視る時と同じで、遠近感はわかりにくいという欠点があります。
上記のコインであれば、レンズ中心部では下記のように見えます。

 

コイン

 

豊富なレンズから選べるので、低倍率から高倍率(2000倍超えまで)まで対応できます。
また、モニタ観察になるので、初めての方でも簡単に観察でき、疲れにくく、長時間の観察(検査)に向いています。

 

真上からの観察(直視)なので、位置決めや寸法測定にも向いています。
PCとの親和性もよく、映像の保存・画像処理・焦点合成 等 様々なソフトウエアが使えます。

 

 

 

 

2. 倍率の違い

実体顕微鏡とマイクロスコープの倍率は単純に比較ができません。
実体顕微鏡は目で確認するのに対して、マイクロスコープはモニタで観察します。

その時にモニタ倍率を含んでしまいます。

 

倍率での単純比較はできませんが、観察視野で比較すると単純な比較ができます。

 

例えば、
顕微鏡の観察視野は視野数で計算します。
視野数20の顕微鏡の場合、10倍時に直径20mmの視野になります。

YM0745-L-2023カタログ切り抜き

 

上記の実態顕微鏡と同じ視野を観察したい場合、下記のマイクロスコープであれば20倍になります。

(実体顕微鏡では10倍になります。)

TG200BA-2023カタログ切り抜き

 

また、マイクロスコープの場合は、モニタサイズで変わってしまいます。

詳細は、下記の記事をご覧ください。

 

 

  マイクロスコープの倍率と顕微鏡の倍率の違い

 

 

 

 

 

 

3. 主な用途の違い

・実体顕微鏡向きの用途
 取付け、加工、立体物の検査・観察 等

 

・マイクロスコープ向きの用途
 外観検査、正確な位置決め(芯出し 等)、凹凸の無い寸法測定 等

 

 

 

4. 松電舎のマイクロスコープ

松電舎ではお客様の用途に合わせ、USBマイクロスコープ、4Kマイクロスコープ、ハイビジョンマイクロスコープなどを取り揃えております。用途、倍率、接続方式などに合わせ、各種お選びいただけます。

 

USBマイクロスコープ

USBマイクロスコープ

4Kマイクロスコープ 4K860PT

4Kマイクロスコープ

ハイビジョンマイクロスコープ

ハイビジョンマイクロスコープ

 

Regarding the New Year’s Day Closure for 2023-2024

Shodensha Vietnam extends its deepest gratitude for your unwavering cooperation and support. We seize this opportunity to express our heartfelt thanks and to inform you of the New Year’s closure schedule for Shodensha Vietnam as follows:

● Closure Period: January 1, 2024
● Business Resumption: January 2, 2024 (Tuesday)

 

We sincerely wish you a splendid New Year’s holiday and hope for your continued happiness and prosperity.

 

Yours faithfully,

LOW-COST HIGH SPEED CAMERA CHU135-C-RS / CHU135-B-RS (COLOR/MONOCHROME)

A versatile 1.3-megapixel type, suitable for various scenarios, is now available at a low price.

– Enhance the convenience of high-speed cameras with two included software applications!
– High-speed cameras available at an ultra-low price starting from the 300,000 yen range.
– Effective pixel count of 1280×1024 resolution at 200fps (frames per second), and at 224×224 resolution, the maximum speed reaches 2300fps.
– Abundant options for resolution and frame rate selection.
* Note: Lenses are required separately.

Low-cost GigE high-speed camera (color/monochrome) CHG40-C-RS/B-RS

It can be used in remote locations that were not possible with USB types. The cable can be extended up to 100m!

 

●The cable can be extended up to 100m, which is not possible with USB types!

●Two types of software are included as standard!!
 ・SpeedCapture allows you to record and check the time before and after the trigger

 ・REAL Slow allows you to observe in slow motion without recording

●High-speed cameras at ultra-low prices

●300 fps (frames per second) at a resolution of 640 x 480 (VGA) effective pixels, or up to 800 fps (frames per second) at a resolution of 240 x 180

●Lenses are required separately.

THE DIFFERENCE BETWEEN AN ELECTRON MICROSCOPE AND A STEREOMICROSCOPE

Electron microscopes and stereomicroscopes each carry their own unique benefits and drawbacks. Understanding the specific characteristics of each type is important to use them flexibly.

 

In general, if expressed in English, both can be called “microscopes”. Although in industry there are instances when microscopes are referred to as microscopes, in this case we will explain “microscopes” in terms of single-eyehole digital display machines.

 1. Difference in Characteristics

・Features of Physical Optical Microscopy:

One big difference is that the Entity Optical Microscope is designed with two independent optical systems, creating two independent light paths on the right and left.

 

2光路設計

 

When observing with a physical Optical Microscope, the viewing range from right and left will be different. If you place a coin as follows to observe:

 

コイン

 

コイン

 

When observing, the observer will see both different visions as a single image, similar to how humans have two eyes.

 

The benefit of this is that the object will look like a real space. You can also understand the feeling of near and far, so people doing processing work often need to use Physical Optical Microscopy. The downside is that it requires a bit of technique when using (first-time users may feel strange about the unnatural image).

 

In the case of a Trinocular Optical Microscope, the camera port will contain an image from one side, either the right or left side (the image will also have a tilt effect).

 

Another disadvantage is the inability to observe at high magnification (due to the need for delicate adjustment between the right and left sides during manufacturing). The magnification is usually low and the magnification ratio is not large. Normally, even for general types, magnification is only 10 to 50 times, and in high-end cases, only about 100 times is the limit of physical Optical Microscopy.

 

Furthermore, in long-term work, workers can experience a lot of pressure. Eye widening, vision correction, and personal adjustments by the observer are necessary.

 

Because the field of vision is different between right and left, it is not suitable for precise positioning and two-dimensional measurement. In the above applications, people often use monocular microscopes.

 

単眼顕微鏡

 

 

・Features of Microscope:

Basically, it mainly uses single lens.

マイクロスコープ

 

Just like when people look at objects with one eye, this has the disadvantage of making it difficult to perceive near and far. In the case of the coin mentioned above, when viewed from the center of the lens, the image will look like this:

 

コイン

 

Because it is possible to choose from a variety of lenses, the microscope can accommodate from low to high magnification (exceeding 2000x).

 

Furthermore, with on-screen observation, even new users can easily observe without causing fatigue, suitable for long-term observation (inspection).

Due to the frontal view (upward angle), it is also suitable for locating and measuring dimensions. It is compatible with computers and can use a variety of software such as image storage, image processing, focus fusion, and many other features.

 

2. Difference in Magnification

Magnification between Physical Optical Microscopy and Microscopy cannot be directly compared. While Optical Microscopy is visually confirmed, Microscopy is observed on a screen.

 

At that time, the magnification of the screen must be taken into account.

Although a direct comparison of magnification is not possible, if the comparison is based on the field of observation, a simple difference can be seen.

 

For example, the field of view of a microscope is calculated by the number of fields. For a microscope with 20 fields, when magnified to 10 times, the field of view will have a diameter of 20 mm.

YM0745-L-2023カタログ切り抜き

 

If one wants to observe the same field as in the mentioned physical Optical Microscopy, with the Microscope below, the magnification will be 20 times.

 

(Meanwhile, in Organic Optical Microscopy, the magnification is only 10 times.)

TG200BA-2023カタログ切り抜き

 

Furthermore, in the case of a Microscope, the magnification will vary according to the screen size.

 

For details, see the article below.

 

3. Differences in Primary Applications

・Physical Optical Microscopy is suitable for applications such as:

Installation, machining, physical object inspection/observation, etc.

 

・The microscope is suitable for applications such as:
Check appearance, determine precise positioning (such as shaft placement), measure dimensions without surface deviation, etc.

 

 

4. Shodensha’s microscope

At Shodensha, we have arranged microscopes such as USB Microscope, 4K Microscope, High Definition Microscope, and many more options to suit the needs of our customers. Depending on the purpose of use, magnification, connection method, we provide many choices for you.

 

USBマイクロスコープ

Microscope USB

 

4Kマイクロスコープ 4K860PT

Microscope 4K

 

ハイビジョンマイクロスコープ

Microscope Full-HD