What is camera image size?

The specifications of C mount camera always include “image size” and “image sensor size”.

In addition, this size is the value needed to calculate the field of view (when selecting the lens).

(To calculation of field of view, please refer to “f value of lens”.)

 

 

The use of “image sensor size”

 

 

You can also calculate the size of one pixel.

For example: The image sensor of 1 / 2.5 inch of 1.3 million pixels (1280 X 1024) is:

Horizontal  5.6 mm / 1280 pixels = 0.0044 mm / dot

Vertical    4.2 mm / 1024 pixels = 0.0041 mm / dot.

 

Image Sensor Size Meaning

 

Typically, when referring to monitor sizes, it indicates the diagonal length. However, the size of an image sensor is not measured diagonally.

For instance, in the case of a previously mentioned 1/2.5-inch sensor with 1.3 megapixels, the horizontal dimension is 5.6mm and the vertical is 4.2mm, resulting in a diagonal length of approximately 7mm.

 

However, the actual 1/2.5-inch sensor measures 10.16mm diagonally. This discrepancy originates from the historical representation of imaging tubes using vacuum tubes, where the size indication was based on the diameter of these tubes. Consequently, the size representation of image sensors has been aligned with this convention.

 

The Actual image sensor size 

 

 

 

水平、垂直が 4:3の撮像素子

 

 

 

水平、垂直が 16:9の素子サイズ

 

The practical use of imaging sensor size

The actual utilization of imaging sensor size pertains to the selection of the requisite lens and the calculation of the distance to the subject.

 

(1) In the case of CCTV lenses (fixed focal length lenses)

 

We shall calculate the required lens based on the desired dimensions of the subject to be captured. At this juncture, the essential factors are the “imaging sensor size of the camera” and the “distance to the subject (W.D.).”

 

 

CCCTVレンズ(固定焦点レンズ)の場合

 

 

 

If we aim to ensure a vertical field of view of 300mm at a distance (W.D.) of 1 meter, what focal length lens should be selected? Assuming the use of a 1/2-inch camera sensor, the dimensions are as follows:

Imaging sensor size for a 1/2-inch camera:

 

1/2インチカメラの撮像素子サイズ

 

 

The formula for the f-value is as follows:

f-value = (Distance to the subject (mm) × Vertical size of the imaging sensor (mm)) / Vertical field of view

By substituting the values:

f-value = (1000mm × 4.8mm) / 300mm = 16mm

By selecting a lens with a focal length of 16mm, the desired vertical field of view can be achieved.

The calculation for the horizontal field of view can be done in a similar manner.

By the way, if either the vertical or horizontal field of view is known, the other can be easily calculated without additional computations.

For a square-shaped imaging sensor with a 4:3 aspect ratio, as in this case, the horizontal field of view would be 300mm × 4/3 = 400mm.

 

(2) In the case of macro lenses 

In the case of macro lenses used for capturing magnified images of subjects, the focal length is typically predetermined. In such instances, the f-value alone does not adequately represent the lens characteristics. Instead, the optical magnification is used as a measure.

For macro lenses, the optical magnification expresses the desired level of enlargement. With a lens at 1x (1:1 magnification), the observed field of view matches the size of the imaging sensor.

For example:
– For a 1/3-inch sensor, the dimensions are 4.8mm × 3.6mm.
– For a 1/2-inch sensor, the dimensions are 6.4mm × 4.8mm.

Considering these specifications, I recommend referring to our company’s macro lens specification sheet for more detailed information.

 

マクロレンズ

 

 

Additionally, when using a macro lens, the imaging sensor size is also utilized in calculating the overall magnification (the final magnification displayed on the monitor). The imaging sensor size plays a crucial role in determining the effective magnification of the captured image.

 

For more detailed information, I recommend referring to the concept of magnification in microscopes, as mentioned in the “Concept of Magnification in Microscopes” resource.

 

USB Camera CS series  USB Camera DN series  GigE Camera 
1/1.1 inch

CS1200-GC(12MP・color) /

CS1200-GB(12MP・monochrome)

 

EG1200-GC(12MP・monochrome)/

EG1200-GB(12MP・monochrome)

1/1.7 inch

CS1200-C(12MP・color) /

CS1200-B(12MP・monochrome)

 

EG1200-C(12MP・color) /

EG1200-B(12MP・monochrome)

1/1.8 inch  

DN3RG-130(1.3MP・color) /
DN3RG-130BU(1.3MP monochrome)

 

DN3RG-200(2MP color ) /
DN3RG-200BU

2MP – monochrome

EG320-C(3.2MP color) /

EG320-B(3.2MP monochrome)

 

EG600U-C(6MP color)/

EG600U-B(6MP monochrome)

1/2.3 inch   DN3R-1000(10MP color)  
1/2.5 inch CS500-C(5MP color) /
CS500-B(5MP monochrome )
DN3R-500(5MP color)  
1/2.9 inch

CS41-C(0,04MP・color) /

CS41-B(0,04MP・monochrome)

 

EG41-C(0,04MP・color) /

EG41-B(0,04MP・monochrome)

1/2 inch

CS130U-C(1,3MP・color) /

CS130U-B(1,3MP・monochrome)

 

CS500U-GC(5MP・color) /

CS500U-GB(5MP・monochrome)

 

EG130U-C(1,3MP・color) /

EG130-B(1,3MP・monochrome)

1 inch

CS2000-C(20MP・color) /

CS2000-B(20MP・monochrome)

 

EG2000-C(20MP・color) /

EG2000-B(20MP・monochrome)

2/3 inch    

EG501-C(5MP・color) /

EG500-B(5MP・monochrome)

Summary:

The imaging sensor size is a crucial value required for calculations such as “field of view” and “lens selection.” In the case of CCTV lenses, the f-value can be computed as follows to determine a lens that can achieve the desired vertical field of view:

f-value = (distance to the subject (mm) × vertical size of the imaging sensor (mm)) / vertical field of view.

For macro lenses, the imaging sensor size is utilized in calculating the overall magnification (the final magnification displayed on the monitor).

 

What is the flicker phenomenon?

The phenomenon of flickering” refers to irregularities observed in footage captured by a camera, such as “repeated variations in brightness” and “shifts in color tone,” which are not perceptible to the naked eye.

In this article, we will provide an overview of “flickering phenomenon” and discuss methods to prevent it.

 

(1) WHAT IS FLICKERING?

 

 

The phenomenon known as “flickering” occurs with certain lighting devices that either flicker in sync with the power frequency or pulse in a dimming system.

Although the human eye cannot perceive the flickering of light, when the camera’s shutter speed is faster than the frequency of the flicker, it results in irregularities in the captured footage, such as “repeated variations in brightness” and “shifts in color tone.” This is referred to as the flickering phenomenon.

Flickering occurs under lighting conditions that involve periodic flashes, such as fluorescent lights, while it does not occur with direct current lighting.

There are two types of symptoms associated with flickering phenomenon:

1. In cameras with a global shutter, the entire image experiences cyclic changes in brightness.
2. In cameras with a rolling shutter, the image exhibits horizontal stripes of brightness variations, and these stripes may appear to flow vertically.

 

 

The phenomenon of “flickering” in cameras with a rolling shutter results in the appearance of horizontal stripes of brightness variations. 

 

 

フリッカー現象

 

 

Methods to prevent the phenomenon of flickering can be categorized into three primary approaches:

1. Utilize lighting devices employing direct current illumination.
2. Adjust the shutter speed to a pace slower than the flicker frequency.
3. Employ a camera equipped with the “flicker-free feature.”

 

 

  1. Utilize lighting devices employing direct current illumination.

Flickering occurs in the presence of lighting sources that exhibit periodic emissions, while it does not occur when using lighting fixtures that operate on direct current illumination.

2. Adjust the shutter speed to a pace slower than the flicker frequency. 

 

The phenomenon of flickering can be somewhat mitigated by adjusting the exposure time or shutter speed settings of the camera, but it cannot be completely eliminated.

Even if it appears to have disappeared, there are still variations in brightness occurring at intervals of several tens of seconds or longer.

In particular, when capturing images with a high-speed camera under fluorescent lighting, significant flickering phenomena occur.

* If it is not possible to adjust the shutter speed on the camera, one can compensate for the reduced illumination by narrowing the aperture of the lens and using lighting fixtures with direct current illumination. This helps to comparatively reduce the impact of pulse lighting.

 

 

3. Employ a camera equipped with the “flicker-free feature.”

 

 The “flicker-free feature” enables the camera to automatically detect the flickering of the light source and capture images at optimal moments with minimal impact on brightness and color tones.

To avoid flickering phenomena, it is recommended to use lighting for high-speed cameras that either operate on direct current illumination or synchronize with the camera shutter, such as strobe lighting.

At our company, we offer a range of products including high-speed cameras, lighting specifically designed for high-speed cameras, and strobe lighting. If you are experiencing difficulties with flickering phenomena, please don’t hesitate to contact us for assistance.

What’s resolution?

Resolution means a numerical value indicating of pixels in a bitmap image. It is the fineness of the grid that expresses the image and it is represented how many pixels are divided in 1 inch.

For microscope, each camera, lens and monitor has different resolution.

1. Cameras and lens resolution are the detail of grid that represents the image.

It is a term that expresses the details of objects (screen, printed result, photographic film, etc.) that can define the size of the physical image.

Therefore, the physical resolution means the minimum unit that can be distinguished from the image (pixel, in the case of a printing result or photographic film, a line / space where the image can be resolved by a resolution chart) is determined per unit length of the object. It is expressed by the number that can be included and represents the definition level not the total number.

Camera and len resolution is very important in selecting resolution.

For example: If camera resolution is high and lens resolution is low, the image will be poor in resolution. Please be careful

  1. What is monitor resolution?

Have a look at this. “about monitor resolution

We will also select cameras and lenses. Please feel free to contact our Technical Advisor.

About LAN port connecting GigE camera

GigE camera is connected by LAN port.

However, there is no power supply line in LAN port attached to normal PC.

(Of course, power is necessary to operate the camera.)

 

There are two ways to use GigE camera.

1. Prepare individual separate power supply for camera.

2. Prepare NIC for PoE (Power over Ethernet) interface (LAN port with power supply line).

1.

There is no problem if an AC adapter is attached to the camera. But in order to connect between PC and camera, power support equipment is very necessary.

It is called PoE injector. It can be obtained easily around 10,000 yen.

It is possible to purchase with PC peripherals manufacturer (Elecom, Sanwa Supply, Buffalo etc.).

2.

NIC is short of Network interface card. NIC has the same meaning as the LAN board and indicates an expansion board. As mentioned above, there is no power supply line in the standard LAN port of a normal PC. Therefore, you need to add such an expansion board (card) to the PC.

   

 

What’s Dynamic Range?

When taking pictures by the camera, there are some cases which the part is too bright (halation / overexposed), and the part is too dark (blackened).

Dynamic range is called the range of contrast difference (defense range) which can be concealed at the same time to the edge before the halation (white jump) from black collapse (too dark to nothing). The unit is db (decibel).

The larger dynamic range number, the better camera.

However, when comparing human eyes and cameras, naked eyes are superior even with excellent cameras.

For example, even if you can see it with naked eyes, shooting with a camera may cause halation (white overexposure) or may not be visible.

What’s high dynamic range function?

It is a technique to express a wider dynamic range than normal image.

Reducing the contrast of the original image with large contrast and take a wide dynamic range.

Even with images with differences in brightness and darkness, it is possible to reduce blackout and whiteout.

The following is an excerpt from cameras specifications made by other companies, but WDR (wide dynamic range) has the same function.

 

There is what is called a dynamic range in the camera vocabulary. The dynamic range is the range of the brightest part and the dark part that the imaging element can feel.

 

If it exceeds this range on the bright side, it becomes halation (white jump).

The screen becomes black when exceeding this range in the dark.

 

HDR and WDR are functions that widen this range and reduce the difference between bright and dark areas. It is used for remove halation.

(However, if you use this function, the contrast will be lowered.)

 

For example: If you shoot a picture with differences in light and darkness, it will be like the picture below.

 

Normal function shooting HDR function shooting

 

Our HDTV camera has HDR function.

ハイダイナミックレンジ

By applying this, you can shoot images with reduced halation.

(It is an effect of widening the dynamic range. Although it does not make infinite, it can not be removed completely)

 

Normal function shooting HDR function shooting
   
Normal function shooting HDR function shooting

 

If this HDR function is combined with a filter and V character block, the performance will be further improved.

<HDR function + polarization filter>

<HDR function + White V character block (background)>

inch of Sensor element

The size of the imaging element written in the specification etc. is not the actual diagonal dimension in notation following the “tube diameter” of the imaging tube age.

For example, 1/2 inch CCD means 「A CCD with the same image size as the image size produced by a 1/2-inch imaging tube」

However, the size will be confirmed with this notation.

If it is a 4: 3 elements, the size will be identified as below.

“CCD Camera image size”
撮像素子のサイズ

CAN I BUY MICROSCOPE WITH MONITOR?

We have the “All in one Microscope” integrated between Android tablet with C-Mount Camera and Video Mircoscope with optional 8 inch monitor.

ビデオマイクロスコープ Microscope Video TG70TV
 +
8.0 inch monitor for video Microscope
GR-085TVA

It is possible to attach a small monitor to microscope pillar as belows:

モニタ用アングル モニタ用アングル

Besides that, there is another suitable option.

固定具  

Put it on monitor stage.

 
モニタ用アングル モニタ用アングル

By doing this you can angle the monitor.

 

モニタモニタモニタ 

DO YOU WANT TO USE MICROSCOPE WHEN YOU ARE ABROAD?

Our microscope adopts the AC adapter as power supply.

The AC adapter supports from 100 V to 240 V so you can use it overseas. (Some products are only 100V compatible)

However, please note that the shapes of plugs in each country are very different.

 

A型 <A type>

It is used in Japan and mainly in North America and Central America.

B型 <B type>
It is mainly used in North America and Central America. Even in Japan, it may be used in personal computers and OA equipment.
C型(SE型) <C type(SE type)>
It is used in parts of China and Europe.

※ There are also many other types of plugs. Transformer plugs are available at home electrical outlets.

About overseas supply voltage

  周波数 電圧
Japan 50/60Hz Single-phase 100/200V, Three-phase 200V
U.S.A. 60Hz Single-phase 115/230V, three-phase 230V
South Korea 60Hz Single-phase 110/220V, three-phase 200/220/380V
China 50Hz Single-phase 220V, three-phase 380V
Thailand 50Hz Single-phase 220V, three-phase 220/380V
Vietnam 50Hz Single-phase 220V, three-phase 380V

HOW TO SHOW CROSSLINE?

HD microscope Camera / PC monitor Direct with microscope camera has a standard cross line display function. You can turn on / off the cross line with the switch on the back.

 

For USB microscope camera, it is possible to display multiple cross lines by using optional Viewer software (MeasurePRO / MeasurePRO – Ex).

 

クロスライン
クロスライン

HD microscope /

PC monitor direct with microscope has standard cross line display function

(Both white line and black line can be displayed)

USB microscope optional

Software MeasurePRO /

MeasurePRO – Ex

Multiple cross lines can be displayed!

 

DO YOU WANT A CHEAP MICROSCOPE THAT YOU CAN TAKE ON A BUSINESS TRIP?

“For those in need of a somewhat advanced yet reasonably priced microscope, the DS-PC4 (USB microscope) and DS-70TV4 (video microscope) are available. While their zoom ratio is limited, and auxiliary lens attachment is not supported, they prove to be exceptionally cost-effective microscopes for individuals whose requirements align with their specifications.

 

In the case of the DS-70TV4, when paired with a commercially available video recorder equipped with a monitor, it becomes highly convenient for on-the-go observations, allowing you to observe from virtually anywhere.”

 

 Integrated LED USB Microscope DS-PC4

Intergrated LED

Video Microscope DS-70TV4

DO YOU WANT MICROSCOPE WITH THE CHEAPEST PRICE?

DS – PC (USB microscope) and DS – 70TV (Video microscope) are available for those who need a microscope which is full – scale yet inexpensive to some extent. The zoom ratio is small, auxiliary lens can not be used, but for those who apply to the conditions it is very affordable microscope.

 

低価格マイクロスコープ
低価格ビデオマイクロスコープ

Integrated LED Microscope

 USB 2MP DS-PC

 Intergrated LED

Microscope DS-70TV

 

In order to reduce the price of microscope, we can combine C-mount with CCTV lens and placing a connector in the middle.

 

Below is a 35mm M3514-MP CCTV lens in the C mount video camera GR-i700 and 5mm connector CR-5 in the middle

安価なマイクロスコープ

Below is how to fix the camera ising fixing tool.

(Since you can adjust the focus on the lens side, you do not need a stand like a microscope.)

安価なマイクロスコープ

Below is the observed picture of a coin yen.

Filed of view is 12mmx9mm at magnification 30 times.

 

Cマウントカメラと汎用のCCTVレンズを組み合わせ、その中間に接写リングを入れる方法があります

If the thickness of tconnector is increased, the magnification can be adjusted easily.

Even if it is not a dedicated lens with a certain magnification, it can be used as microscope.

Microscopes Ideal for Use on Inspection Lines

If you are using it on an inspection line, a High-Definition Microscope, Video Microscope, or Auto-Focus Microscope, all of which provide real-time display with no delays, would be suitable.

If you require high-resolution observation in full high definition, a High-Definition Microscope or an Auto-Focus Microscope is the optimal choice. If resolution is not a primary concern, a Video Microscope would be the most suitable option.

ハイビジョンマイクロスコープ

High-Definition Microscope

High-Definition Microscope

Advantages

– HD Microscope has smooth movement and high resolution as well as

– HD Microscope can be connected directly to Full HD Monitor through the HDMI port and industrial monitor as well.

Disadavantages

– HD Microscope can not use with a monitor that does not have Full HD and HDMI port.

   

オートフォーカスマイクロスコープ

Auto-Focus Microscope

Auto-Focus Microscope

Advantages
– No need for manual focusing, thanks to ultra-fast auto-focus.
– Achieves smooth movement and high resolution.

 

Disadvantages
– Limited options for image storage due to direct monitor connection.
– Recording media is restricted to the built-in MicroSD card.

   

ビデオマイクロスコープ

Video Microscope

Video Microscope

Advantages

– If it’s just a video connection, it can be used anywhere. It features smooth movement.

A wide variety of external crises are commercially available.

Disadvantages

– Due to the number of scanning lines of TV monitor, the resolution is quite low.

 

Summary

For ease of use on inspection lines, microscopes with no display delays, such as High-Definition Microscopes, Auto-Focus Microscopes, and Video Microscopes, are suitable choices.

 

If you desire high-resolution observation in full high definition, High-Definition Microscopes and Auto-Focus Microscopes are ideal options. Alternatively, if resolution is not a primary concern, a Video Microscope would be the most suitable choice.

CAN I MEASURE THE LENGTH WITHOUT CALIBRATION?

There are several methods. One of these methods is to draw a cross line on the monitor and measure it by using digital micrometer XY table.

 

Another methos is to measure the length by using software. In that case, the lens, camera, PC monitor and resolution are all set on the same environment

Therefore, if you calibrate at each magnification and save the calibration data first. Then, you can view the data without calibrating.

 

Since our standard variable magnification lens (SDS – M) has the stop key, it is reproducible for anyone using it. It is possible to measure if you already read the calibrated file with that scale before.

Furthermore, with a high-definition non-calibration microscope, calibration values are pre-registered from the outset, and since the camera and lens are always synchronized, there is no need for calibration. This allows for instant measurement even when changing the magnification.

 

クリック付ズームレンズ

Standard variable magnification lens

SDS-M

500万画素USB3.0マイクロスコープ

 5MP USB3.0 microscope

equipped with a zoom lens with a click function

TG500CS

寸法測定(自動校正)マイクロスコープ

The camera and lens are synchronized!

  • Measurement without calibration
  • Instantaneous magnification identification Dimension measurement (automatic calibration) microscope    CT200HD

 

Please contact our Technical Advisor for more details.

MICROSCOPE WITH TELECENTRIC LENS

The telecentric lens is very suitable for measuring the product size at high magnification with no expansion of the object within the depth of focus, less distortion of the surroundings.

 

Since it is a fixed magnification, there is no versatility like a zoom lens.

(There are many types of lens with a fixed magnification)

In addition, Since the magnification does not change, we only need to calibrate 1 time in order to measure.

HOW TO OBSERVE THE SIDE VIEW OF THE OBJECT WITH MICROSCOPE?

There are cases where you want to change the microscope to side view by observing the inside of a slightly thicker tube or solder observation of BGA etc. (I want to observe the side).

In that case we will introduce useful things.

Its product called reflective mirror box.

反射ミラーボックス  

There are 2 following suitable lens.

The upper part is a magnigying lens, about 25x to 135x (The variable magnification lens will be changed with aperture SDS – M19), the bottom is a variable magnification lens, about 30x to 60x.

2種のレンズに取り付け可能

The reflection mirror box is attached to the lens head.

Mounting brackets are required separately when installing. Please do not hesitate to contact us if you need any furthure information.

反射ミラーボックスはレンズの先端に取付け 反射ミラーボックスはレンズの先端に取付け

However, a connection ring is necessary to attach. (Please contact us for more details)

リング照明が装着不可

It is impossible to attach LED Ring Light when using the reflection morror box

Besides that, the ordinary LED Ring Light can not be installed.

Thus, it is necessary to devise measures such as using fiber lighting, thin lighting, etc.

 

<Slim light>    
薄型照明 薄型照明 薄型照明
     
<Optical fiber light>
Optical fiber light can be matted inside.
ファイバー照明 ファイバー照明

CAN I SEE NANO-ORDER?

There is a limit to what can be seen with visible light. Light has a wavelength (length), and those shorter than the wavelength of light can not be seen by the eye. There are limits of optical microscope / microscope there.

Therefore, an electron microscope using an electron beam (electron beam) whose wavelength is shorter than visible light.

 

In the past, there were only 10 million yen or more for digital micrometer microscope. Right now, some digital micrometer were about 6 million yen.

 

In various regions, there are public industrial testing laboratories, some of which offer testing services and equipment usage with electronic microscopes.

 

※Please note that we do not handle electron microscopes.

WHAT IS THE DIFFERENCE BETWEEN A SIMPLE XY TABLE AND DIGITAL XY TABLE?

It is a support table (sample table) that can be moved in both X and Y directions. It is advantageous when using the appropriate position adjustment becomes easier.

 

A simple XY table that can move the stage with a coarse movement knob.

A simple XY table that can move the stage with an auxiliary knob. Xy table can move in large space and wide range. In addition, XY table can move quicky since the amount of movement per dial rotation is high.

 

A digital micrometer XY table is a XY table attached to the microscope in both X and Y directions. XY stage can move precisely with fine movement by turning the knob of the digital micrometer. The length can also be measured from the photographed image and the numerical value of micrometer. It is particularly effective for measuring sizes that can not be measured with image measurement software. Also, since the digital micrometer is capable of issuing calibration certificates, it is suitable for high precision measurement of length.

Although it can move precisely, on the other hand, it moves only 0.5 mm in one turn. Therefore, you can not move quickly.

 

簡易XYテーブル(落射照明タイプ) TK100 Simple XY table TK100
デジタルマイクロメータ付きXYテーブル Digital micrometer XY table TD100
デジタルマイクロメータ付きXYテーブル Digital micrometer XY table TD100 can measure length with high accuracy

 

If you need any furthure information, please don’t hestitate to contact our Technical Advisor.

WHAT’S VIGNETTE?

What is vignette?

 

A black part like a black tunnel which occurs when using an incompatible lens with image ssensors. It looks like it peeps in the tunnel.

ケラレ

See the dark side in the 4 corners of the frame

 

Cause of VIGNETTE

For example, in the case of a borescope,
As the sensor size of the camera connected to the borescope becomes larger, the vignetting area becomes wider. On the other hand, the image seen directly with the naked eye becomes closer to the image observed through the borescope.

 

In the case of a microscope
Typically, microscope cameras are designed not to have vignette (shadows in the four corners). Designers make allowances to prevent vignette, but the degree varies depending on the manufacturer.

WHAT’S MEGAPIXEL?

Megapixel means 1.000.000 pixels.

Megapixel camera refers to cameras with more than 1 million pixels.

Besides that, there are also lens that corresponds to Megapixel.

If you choose the lens that matches with the number of pixels of the camera, the picture will be better.

The balance between camera and lens is very important.

If you have any requirements on camera and lens, please don’t hestitate to contact us.

WHAT’S SCREEN RESOLUTION?

Pixel density in a bitmap image refers to the numerical representation of the granularity of the grid that expresses the image, indicating how many divisions there are in one inch.

 

In the case of a microscope, each of the camera, lens, and monitor has its own resolution.

 

1. Camera and lens resolution

Camera and lens resolution refers to the granularity of the grid that expresses the image.

 

It is a term that represents the detailing capability for subjects (such as screens, print results, photographic films, etc.) where the physical size of the image can be defined.

 

Therefore, physical resolution expresses the ability to distinguish the smallest unit of that image (pixels on a screen, resolution lines/spaces on print results or photographic film) per unit length of the subject, and it represents the level of detail rather than just the total count.

 

When selecting a microscope, especially the resolution of the camera and lens is crucial. For instance, if the camera has high resolution but the lens has low resolution, the resulting image will have poor resolution. Therefore, please be mindful of this aspect when choosing a microscope.

 

2. Monitor resolution

 

Monitor resolution refers to the ‘total number of pixels used for display,’ such as 1024×768.

 

XGA: 1024×768 pixels

SXGA: 1280×1024 pixels

VGA: 640×480 pixels

 

Description

 

Resolution

 

Monitor ratio

VGA

640×480

4:3

SVGA

800×600

4:3

XGA

1024×768

4:3

WXGA

1280×768

15:9

WXGA

1280×800

16:10

SXGA

1280×1024

5:4

UXGA

1600×1200

4:3

FHD (Full-HD, 1080p)

1920×1080

16:9

 

If you are using our PC Monitor Direct Microscope, Video Microscope, and High-Definition Microscope, the monitor aspect ratio is crucial.

 

For the PC Monitor Direct Microscope TG80XGA3 and all Video Microscope models, please use a monitor with a 4:3 aspect ratio or a widescreen monitor that can switch to a 4:3 aspect ratio.

 

For the High-Definition Microscope, use a monitor with Full High-Definition support (1080p) and for the 4K Microscope, use a monitor with 4K support and a 16:9 aspect ratio, or the displayed images may not have the correct proportions.

 

For USB microscopes, you can use them regardless of the monitor’s resolution. The USB camera’s image sensor has a 4:3 aspect ratio, so even if the PC screen has a different aspect ratio, the image within the launched application will be in a 4:3 format.

How to choose a Digital microscope?

The selection criteria for microscopes can be broken down into three main steps:

 

 

STEP 1: Basic System Selection

1. Magnification (Field of View): Consider the range of magnification options based on the desired field of view.
– Low Magnification: 5–50x (Approximately 67.5 x 51.0mm to 7.0 x 5.3mm field of view)
– Medium Magnification: 20–140x (Approximately 17.5 x 13.2mm to 2.7 x 2.0mm field of view)
– High Magnification 1: 40–240x (Approximately 9.0 x 6.7mm to 1.4 x 1.0mm field of view)
– High Magnification 2: 70–800x (Approximately 4.5 x 3.2mm to 0.4 x 0.3mm field of view)
– Ultra-High Magnification: Beyond 1000x (Approximately 1.5 x 1.1mm to 0.12 x 0.09mm field of view)

2. Working Distance: Consider the distance from the lens tip to the object, which is fixed for zoom lenses but varies with magnification.

3. Display Speed: Choose between High-Definition (HD) for smooth real-time imaging and USB for various additional features.

 

 

STEP 2: Resolution Selection

– HD Type: Choose between Full HD (1920×1080) or 4K (3840×2160) resolution.
– USB Type: Select from cameras with resolutions such as 1.3 million pixels (CMOS) or 5 million pixels (CMOS), considering factors like real-time display and data transfer speed.

 

 

STEP 3: Lighting Selection

– Ring Lighting is the basic option, but for higher magnifications, choose between Ring Lighting and Coaxial Lighting.
– Additional options like Transmitted Lighting, Dome Lighting, and Low-Angle (Darkfield Observation) Lighting are available.

 

 

Additional Considerations

– Explore optional accessories for diagonal observation, polarized observation, height measurement, auxiliary lenses, rear converters, filters, and other optical options based on specific needs.

 

 

Conclusion

 

Microscope selection involves considering magnification, working distance, display speed, resolution, and lighting. Evaluate these factors to meet specific requirements and applications.

Types of microscopes used for observing oral bacteria

Phase contrast microscopy is used for observation of oral bacteria including periodontal disease bacteria.

Phase contrast microscope is a biological microscope that can observe by converting the phase difference of light into contrast.

You can observe specimens close to transparent bodies without dyeing.

 

By using a normal phase contrast microscope you can observe bacteria in the oral cavity.

 

There seems to be a company specialized especially for dental clinics especially among them.

http://www.ptech.jp/pscope/(web site in Japanese)

http://www.ipona.biz/dentist/dentist.html(web site in Japanese)

Confirmation points if you thought that the light intensity of metallic microscope is dark

Metallic microscopes have more adjustment points than ordinary microscopes. Depending on the adjustment, the light intensity may decline extremely. If it is dark (or no light comes out) please check as the following instructions.

 

Polarization observation change lever

When both levers protrude, the light path is blocked.

   

Be make sure to switch between polarization observation and normal observation.

   
■Optical axis adjustment of light source

If the optical axis and the tilt of the light source deviate greatly, the light irradiated from the objective lens may be drastically declined.

   
■Adjusting the aperture in the middle of the optical path  

There are two aperture functions in the middle of the light path.

If this is narrowed too much, the irradiated light may decay considerably.

 

How to connect microscope camera to C mount?

The microscope camera has a connector len at the top.

Therefore, you can attach to the eyepiece part of the microscope and the JIS lens.

connect to eye piece part connect to Trinocular lens barrel
(camera port)

 

You can also connect to the C mount by removing the connector lens at the tip.

Camera for the microscope consists of the three parts of the belows photo.

 

  1. Camera body 2. C mount ring 3. Connector lens

Remove only the connector lens, be make sure to attach the C mount

 

In this picture, you can connect as a C  mount camera.

 

In this case, the lens is not attached to the camera. Therefore, you need to adjust the field of view on the C mount side (microscope side).

For details, please refer (“Connection of camera for microscope”).

Matching the focal point of the naked eye observation and the focal point of the microscope camera

Normally, focus adjustment of the microscope is performed when observe by naked eye.

Due to individual variations of the camera, the focal point of the naked eye observation may not match the focus of the camera.

In the case of JIS lens (see “Connecting camera for microscope”), the back focus is attached to the lens.
You can adjust easily.

 

In case of C mount, some of the microscope side C mount part has back focus, but it is very slight.

There is also a mechanism to adjust the back flange (back focus in this case) on the camera side. However, I think that this is also numerically small.

 

What is an eyepiece micrometer (reticle)?

Eyepiece micrometer (reticle) is a glass scale used by incorporating it in an eyepiece when measuring the size of a specimen with a microscope.

There are various kinds depending on how to use it.

Corresponding size varies depending on the size of the microscope.

φ19, φ20, φ20.4, φ21, φ24, φ25, φ26, φ27 mm are general-purpose sizes and can be easily purchased with onlineshops.

For many eyepieces, you can insert later as needed.

レチクル

Since there are some errors in the magnification of the objective lens, the measurement of eyepiece micrometer (reticle) becomes simple.

<How to use>

The width of the observed objects on a bar divides will be changed depend on the magnification of the objective lens.

Caculation:

Width on specimen of 1 scale (mm) = actual size of 1 scale of micrometer / magnification of objective lens

<Example>

Using a micrometer (0.1 mm / scale) of 10 mm / 100,

When a lattice sample of 0.2 mm pitch was measured with a microscope has objective magnification of 10 times

0.1 mm (actual scale of 1 scale) / 10 times (objective magnification) = 0.01 mm (1 scale on sample)

 

 < Measurement 10mm/100 (reticle) has step 0.1mm>

 

レチクル レチクル
< Measurement 10mm/100 (reticle) has step 0.1mm>

 

Measure sample of 0.2 mm lattice

 

レチクル レチクル

 

In 0.2 mm has 20 scales. (Precisely 19.5 scales)

The difference (about half scale) is due to the magnification error of the objective lens.

CAMERA CONNECTION FOR MICROSCOPES

The camera port of the microscope is roughly divided into the following two types: JIS lens and C mount

(Besides that, there are other classic or foreign types)

JIS lens

(straight lens diameter of 23.2mm)

C-mount

(Screw M25.4mm)

   

If you want to connect a camera with a “C-mount” to the mount of a microscope, there are two methods:

Convert the camera port on the microscope side to C-mount:
If the microscope already has a C-mount, you can directly attach the camera to it.

However, if the microscope has a JIS (Japanese Industrial Standard) microscope tube, you will need to modify the port on the microscope side.

Please note that not all microscopes allow for port conversion, so it may not be possible in some cases.

 

 

 

 

The biggest advantage of this camera is to be connected to the eyepiece section.

Cマウント

 

 

 

 

<Camera for microscope attached to C mount>

C mount is one of mount camera standards. (Please refer to “C mount, CS mount”)

Because of mount camera standards, a microscope camera is unnecessary.

It is the greatest merit that C mount type general purpose camera can be connected as it is. (Can not be connected to eyepiece section.)

 

 

However, the field of view (magnification) will change depending on the size of the image sensor on the camera.

As there is no lens on the camera, the magnification can not be adjusted.

(It must be selected when purchasing the camera adapter for microscope)

The following picture is a system diagram of some microscope manufacturers.

C-mount has various types from 0.25X to 0.63X.

It is necessary to purchase C mount matches to the number of inches of C mount camera.

Since it’s all C mount, the focus themselves matches. However, the field of view will be narrow or vignetting (shadows of four corners of the image) occurs.

 

2.  Make a C-mount camera compatible with a JIS (Japanese Industrial Standard) microscope tube

JIS鏡筒

When you attach a JIS microscope tube adapter lens to your camera, you can mount it onto the JIS microscope tube.

The biggest advantage of this camera is that it can also be connected to the eyepiece lens section.

If you remove the front lens, you can also connect it to a C-mount. Please refer to the instructions on “How to Connect a Microscope Camera to a C-mount” for reference.

3. Field of View Considerations:

 

Camera internal sensors (CMOS or CCD) come in various sizes. Ideally, the lens magnification should be adjusted according to the sensor’s size.
If the sensor size is 1/2 inch, it is recommended to choose a lens with approximately 0.5x magnification.

 

 

Cマウント JIS鏡筒

If you have other inch sizes or specific requirements, please contact our technical support.

 

Field of view when taking Full HD photograph by microscope

High definition video will be horizontally image of 16: 9. (1920 X 1080)

 

The field of view of the stereo microscope is a round view.

 

To take pictures of a microscope with a Full HD camera, there are two following methods.

1.

If shooting all fields of view of the microscope, put out small images on the monitor.

(The resolution will be about ¼)

    2.

If shooting to avoid dark conrner,

It will be smaller than the actual field of view.

(In fact, it is enlarger)

it will be smaller than the field of view

 
     
Field of view observe with the naked eyes   HDCE-20T
Full HD camera images for the HDCE-20T microscope
 
     

 

If you want to extend the field of view, you should use a 4: 3 camera (PC monitor direct with (analog RGB) camera or video camera) and se a len with a wide field of vision. (Some vignette will come out.)

 

Below picture is microscope camera with X 0.36 lens attached to our PC monitor direct (analog RGB) camera.

* Shadows appear in the four corners. Resolution is also lower than Full HD.

 

Use a 4: 3 camera and a wide field of vision len, the image likes the picture below.

How to save observation images with a microscope?

I will explain the field of view of a camera of microscope attached to an eyepiece lens or JIS lens.

(For camera of microscope which C mount is attached, please refer to “Connection of microscope and C mount camera”.

 

The left picture shows the image using microscope with the naked eye.

1. Camera of microscope  

Normally, cameras for microscope are made in order to avoid vignettes (shadows of the four corners)

It is the red frame of the top.

Although it designs with margin in order to avoid vignetting, its level of variation depending on the manufacturer.

However, it is enlarged more reliably than the naked eye observation.

Because the magnification of the lens of camera for microscope is fixed, it is not possible to change the field of view.

   
2. Commercial video camera and connector lens

I am observing it by installing a connector len on a commercially video camera.

Commercially video cameras have a zoom function.

Using this zoom function, we adjusted the field of view.

Vignetting (shadow of four corners) occurs, but you can take a wide field of view. (Yellow frame of the top photo)

You can export output signal to the monitor using the HDMI cable from the video camera.

HDMI Cable

BASIC ADJUSTMENT OF MICROSCOPE

Firstly, please check the proper eye position. This eye position is called eye point.

With the old microscope, the eyelashes were close to touch the eyepiece. Now, there are various high eye point that you can observe while wearing glasses.

 

If the eyepiece contains the following marks, it is a high eye point.

 

I will show you one way to easily know eye points.

Place copy paper on the eyepiece.

Changing the distance of the copy paper from the eyepiece changes like the photo below.

The point with the most focus is the eye point.

< Focus status> < Unfocus status>

Place your eyes at the correct position (eye point) then adjust.

1.Inter-eye adjustment (Adjust the eyepiece and eye width.)

When looking at the binocular microscope, there is one tip is to look far away.

Looking into the eyepiece while consciously looking far away then adjust the width of both eyepiece lenses.

 

I looked through the eyepiece with both eyes and the adjustment is complete once the field of view becomes one full circle.

2.Diopter adjustment

Adjust by turning the diopter adjusting ring so that both eyes are in focus.

(One-sided diop adjustment) (Two-sided diop adjustment)

3.Zoom adjustment

(1) Turn the zoom dial to set the minimum magnification and focus.

(2) Next, turn the zoom dial to set the maximum magnification and focus.

(3) Return to low magnification again. Adjustment is completed if the focus is on the low magnification side.

When the focus is out of focus

Repeat steps (1) and (2) for one-sided diop adjustment.

If it is a two-sided diop adjustment, turn the diopter adjusting ring so that the two eyes are in focus at the stage (3).

Then repeat the operations from step (1) to (3).

What’s is Gige Camera?

GigE camera is short of Gigabit Ethernet camera.

Expensive dedicated board is not required, general-purpose Ethernet cable can be used for the cable.

The connection port is also LAN port.

However, a power supply for driving the camera is necessary. (See “LAN port connecting GigE camera”)

 

 

 

 

 

The cable can be extended up to 100 m.

Because it is cheap and can communicate at high speed, it is a user-friendly camera that can construct an inexpensive image processing system.

 

The price is equivalent to USB cameras, but the feature of this product is the ability to extend the cable up to 100 meters. Additionally, it offers stable communication.

 

The initial setup (environment configuration) is slightly more complex than USB cameras, but once connected, the communication stability surpasses that of USB.

 

About Image Size of Camera

Cマウントカメラの仕様書にはかならず、「イメージサイズ」、「撮像素子サイズ」が記載されています。
同じレンズを装着した場合、素子サイズが小さい方が視野が狭くなります。
また、このサイズは、視野の計算(レンズの選択)時に必要な値となります。
(視野の計算方法は「レンズのf値」を御参照ください。)

1画素のサイズもこれで計算できます。
例えば、130万画素(1280 X 1024)の1/2.5インチの撮像素子であれば
水平方向  5.6mm/1280画素=0.0044mm/dot
垂直方向  4.2mm/1024画素=0.0041mm/dot   となります。

ちなみに、レンズ側の仕様書にも、「1/2インチカメラ対応」等の記載があります。
この場合、「1/2インチカメラだけに対応している」という意味ではなく、「1/2インチ以下のカメラならば使える」という意味です。
但し、上にも記載したとおり、視野は変わってしまいます。

弊社が主に使っている  1/2インチ、1/2.5インチ、1/3インチ 撮像素子のサイズは下記の通りです。

撮像素子のサイズ

不思議なことに気が付きませんか?
例えば1/2インチ素子の場合、対角が8mmになっています。
1/2インチは12.7mmであるにも関わらず8mmです。
これは撮像素子の特殊な事情で、撮像管時代の基準がそのまま使われているためです。

What is S Mount?

S-Mount với kích thước nhỏ và giá thành thấp.

There is no regulation of flange back.

S-mount are used for compact cameras and board cameras. Currently, there are many lenses with small size and low price.

(例)

 

S → C mount conversion ring is also on sale.

 

C mount has provisions of flange back, whereas S mount does not have flange back specification.

Therefore, even if the aperture is changed, It doesn’t mean that C mount lenses can be used.

When using the C mount lens, I think that it is necessary to try it.

DISTRIBUTION OF METALLURGICAL MICROSCOPES

■Adjust the light source position 金属顕微鏡
(BEFORE ADJUST) (AFTER ADJUST)
金属顕微鏡 金属顕微鏡
   
■ Adjust lens position of the light source 金属顕微鏡
(BEFORE ADJUST) (AFTER ADJUST)
金属顕微鏡 金属顕微鏡
   

■ The image sensor size of camera

Even the image sensor size of camera changes.

Reducing the image sensor size, the influence of variations in illuminance distribution may be reduced. However, its picture will be enlarged than the actual naked eye observation.

Camera sensor 1/2 inch  Camera sensor 1/3 inch
金属顕微鏡 金属顕微鏡
(Note) Since the cameras are different, color and contrast are slightly different.

 

How to see large objects with a microscope?

With normal microscope, the distance from the lens center to microscope stand is limited. Thus, if it is a wide object, it will hit the stand.

 

At our company, we have options (special order) like the theone on the right picture.

顕微鏡
   

This option can be installed both vertically and horizontally on our large stand.

顕微鏡 顕微鏡
   
■Set up on the long side  
顕微鏡

note: When using in this direction we recommend attaching a weight (optional) to stabilize

   
■Set up on the short side  
顕微鏡

 

Proposal for fluorescence (GFP) filter equivalent

Considering the filter that cuts the wavelength of illumination (470 nm, 505 nm) and allows only the emission wavelength to pass. It will be below.
GFPフィルター

SC 56 is Fuji Film’s model number.

The equivalent one is model O56 of HOYA company.

We can install it to our stereo microscope too.

GFP

It is not specified as GFP, but the characteristics are equivalent.